DGTP PCR GRADE

Code: 3732703001 D2-231

Application

2′-Deoxyguanosine 5′-triphosphate trisodium salt is utilized for methods such as DNA sequencing, PCR and other DNA polymerase based DNA synthesis and ...


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€908.70 EACH
Discontinued
€1,117.70 inc. VAT

Application

2′-Deoxyguanosine 5′-triphosphate trisodium salt is utilized for methods such as DNA sequencing, PCR and other DNA polymerase based DNA synthesis and repair techniques.

General description

2′-Deoxyguanosine 5′-triphosphate (dGTP) is made of a guanine nucleobase attached to deoxyribose and a 5’;-hydroxyl on the sugar linked to a chain of three phosphate residues. It is used by the cells for the synthesis of DNA with the help of DNA polymerase.

dGTP, PCR Grade, is a 100 mM clear, colorless solution of the sodium salt (pH 8.3). PCR Grade nucleotides from Roche are specially manufactured and purified to the highest possible chemical purity. PCR Grade nucleotides contain at least 99% of the relevant deoxynucleoside-triphosphate (dNTP) and less than 0.9% deoxynucleoside-diphosphate (dNDP).

Other Notes

For general laboratory use.

Physical form

solution of dGTP in water (pH 8.3).

Preparation Note

Working solution: We recommend to prepare a deoxynucleotide mix containing dATP, dCTP, dGTP, dTTP (10 mM, each); (e.g., for the preparation of 100 µl nucleotide mix add 10 µl of dATP, dCTP, dGTP, dTTP (each) to 60 µl Water, PCR Grade).

Quality

Function tested in RT-PCR. Tested for absence of contaminating deoxyribonucleases, ribonucleases and nicking activities according to the current Quality Control procedures.

assay﹤0.9% (dNDP), 99% (dNTP)
concentration100 mM
formsolution
InChI keyIWGGLKOTEOCWQP-BIHLCPNHSA-K
InChI1S/C10H16N5O13P3.3Na/c11-10-13-8-7(9(17)14-10)12-3-15(8)6-1-4(16)5(26-6)2-25-30(21,22)28-31(23,24)27-29(18,19)20;;;/h3-6,16H,1-2H2,(H,21,22)(H,23,24)(H2,18,19,20)(H3,11,13,14,17);;;/q;3*+1/p-3/t4-,5+,6+;;;/m0.../s1
manufacturer/tradenameRoche
packagingpkg of 1,250 µL (11969030001 [100 mM]), pkg of 4 × 1,240 µL (03732703001 [4x100 mM]), pkg of 250 µL (11934538001 [100 mM])
Quality Level100
SMILES string[Na+].[Na+].[Na+].NC1=Nc2c(ncn2[C@H]3C[C@H](O)[C@@H](COP([O-])(=O)OP([O-])(=O)OP(O)([O-])=O)O3)C(=O)N1
storage temp.−20°C
usagesufficient for 125,000 standard reactions (03732703001), sufficient for 6,250 standard reactions (11934538001), sufficient for 31,250 standard reactions (11969030001)
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